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V5-tag Antibody [HRP], pAb, Rabbit

Cat. No.	Name	Size	Price
A00877-200	V5-tag Antibody [HRP], pAb, Rabbit	200 μg(5x40μg)	$248
A00877-40	V5-tag Antibody [HRP], pAb, Rabbit	40 μg	$55

Full Name

V5-tag Antibody [HRP], pAb, Rabbit

Abbreviated name-1

Rabbit Anti-V5-tag [HRP] pAb;

Documents
Document-MSDS: 8303_20081118024913.PDF (PDF)
Document-MSDS: 9195_20090114012119.PDF (PDF)
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Reference

Description

GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.

Concentration

1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Immunogen

V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH

Purification Method

Immunoaffinity chromatography

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Conjugation

Horseradish peroxidase (HRP)

Host Species

Rabbit

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

Related Products

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