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Cat. No.
 Name
Size   
Price
 Selected
A00877-40
40 μg
$87
A00877-200
200 μg(5x40μg)
$300
A00877-100
100 ug
$218
V5-tag Antibody [HRP], pAb, Rabbitcustom antibody production
V5-tag Antibody [HRP], pAb, Rabbit
Full Name V5-tag Antibody [HRP], pAb, Rabbit
Abbreviated name-1
 Rabbit Anti-V5-tag [HRP] pAb;
Documents
V5-tag Antibody [HRP], pAb, RabbitDocument-MSDS: 8303_20081118024913.PDF (PDF)
V5-tag Antibody [HRP], pAb, RabbitDocument-MSDS: 9195_20090114012119.PDF (PDF)
Figures
V5-tag Antibody [HRP], pAb, Rabbit zoomV5-tag Antibody [HRP], pAb, Rabbit
Reference
Description
 GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
Ig Subclass
 Rabbit IgG
Storage
 The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
 GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Concentration
 40μg:1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
100 μg:0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Immunogen
 V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Purification Method
 Immunoaffinity chromatography
Reconstitution
 Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Applications
 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Conjugation
 Horseradish peroxidase (HRP)
Host Species
 Rabbit
Technical Manual
 ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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