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Nicastrin Antibody, pAb, Rabbit

*This product has been discontinued!*
Nicastrin is a Type I transmembrane glycoprotein. Along with presenilin, APH-1, PEN-2, it comprises the multimeric gamma-secretase complex. The gamma-secretase complex can cleave the beta-amyloid (A4) precursor protein and yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of nicastrin expression results in mouse embryonic lethality.GenScript Rabbit Anti-Nicastrin Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, FTRDLMEKLKGRTSRIAGLAVSL, corresponding to amino acids 103-125 of the N-terminus of human nicastrin.Rabbit Anti-Nicastrin is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.
A00883
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Overview
Specificity GenScript Rabbit Anti-Nicastrin Polyclonal Antibody can detect endogenous level of human nicastrin. Predicted to react with mouse and rat nicastrin according to sequence homology.
Host Species Rabbit
Immunogen Synthetic peptide (KLH-conjugated) corresponding to amino acids 103-125 (FTRDLMEKLKGRTSRIAGLAVSL) of the N-terminus of human nicastrin
Species Reactivity Human
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Applications
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Other applications User-optimized
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Properties
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Purification Immunoaffinity chromatography
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Background
Target Background Nicastrin is a Type I transmembrane glycoprotein. Along with presenilin, APH-1, PEN-2, it comprises the multimeric gamma-secretase complex. The gamma-secretase complex can cleave the beta-amyloid (A4) precursor protein and yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of nicastrin expression results in mouse embryonic lethality.
GenScript Rabbit Anti-Nicastrin Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide, FTRDLMEKLKGRTSRIAGLAVSL, corresponding to amino acids 103-125 of the N-terminus of human nicastrin.
Rabbit Anti-Nicastrin is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 0.5 mg/ml.
Synonyms Rabbit Anti-Nicastrin pAb;
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Citations
Kaushik G, et al. Honokiol inhibits melanoma stem cells by targeting notch signaling. Mol Carcinog. (2014-12)