V-ATPase Subunit B 2 Antibody, pAb, Rabbit

*This product has been discontinued!*

GenScript Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B.Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.

Cat. No.	A00960
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	GenScript Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B.Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.
Host Species	Rabbit
Immunogen	Synthetic peptide (KLH-coupled) within residues 300-350 of human V-ATPase Subunit B2 protein (Swiss Prot: P21281)
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.03-0.125 µg/ml
Western Blot	1.0 µg/ml
Other applications	User-optimized

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Properties

Form	Liquid
Storage Buffer	1×PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Concentration	0.5 mg/ml
Storage Instructions	The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable
Note	Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.

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Background

Target Background

The V-ATPase subunit B 2 is related to a gene that encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain.The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunits.The V1 domain contains the ATP catalytic site.The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.GenScript Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide conjugated to KLH.

Synonyms

Rabbit Anti-V-ATPase Subunit B 2 pAb;

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Huikyong Lee, et al. Roles for H /K -ATPase and zinc transportor 3 in cAMP-mediated lysosomal acidification in bafilomycin A1-treated astrocytes. Glia. (2020-12)
2. Ahmad A, et al. Role of Vacuolar ATPase in the Trafficking of Renal Type IIa Sodium-phosphate Cotransporter. Cell Physiol Biochem. (2011-06)