Histone H4 Antibody, pAb, Rabbit
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Histone H4 Antibody, pAb, Rabbit  |
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| Full Name |
Histone H4 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-Histone H4 pAb; |
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Description |
Histone H4 is a core component of nucleosome facilitating nucleosome wrapping and DNA compacting into chromatin. This limits the DNAs accessibility to the cellular machineries for which DNA is required as a template. Histones thereby play a central role in transcription regulation, DNA repair and replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, of which the process is called the histone code, and nucleosome remodeling.
GenScript Rabbit Anti-Histone H4 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to the residues of human Histone H4 protein. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Histone H4 Polyclonal Antibody detects endogenous levels of human Histone H4 protein. It is predicted to react with Histone H4 protein in mouse, rat, bovine, fruit fly, maize, baker's yeast according to sequence homology. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) derived from human Histone H4 |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Predicted Band Size |
15 kD |
Observed Band Size |
15 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may depend on secondary antibody affinity, antigen concentration, detection method, temperature, incubation length, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 7215_20081023030535.PDF (PDF)