ERK2 Antibody, pAb, Rabbit

*This product has been discontinued!*

ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases.GenScript Rabbit Anti-ERK2 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide (KRIEVEQALAHPYLEQYYDPSDE) corresponding to 300-322 residues of human ERK2 (Swiss Prot: P28482).

Cat. No.	A01194
Size	Buy in bulk

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Overview Properties Background

Overview

Specificity	GenScript Rabbit Anti-ERK2 Polyclonal Antibody detects endogenous levels of human, mouse and rat ERK2 (p42 MAP Kinase) and slightly cross-reacts with ERK1 (p44 MAP Kinase).
Host Species	Rabbit
Immunogen	KLH-coupled synthetic peptide (KRIEVEQ ALAHPYLEQYYDPSDE) corresponding to 300-322 residues of human ERK2 (Swiss Prot: P28482)
Species Reactivity	Human, mouse, and rat. This product has not been tested with other species yet.

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Properties

Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Purification	Immunoaffinity chromatography

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Background

Target Background

ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) includes two closely related isoforms ERK1 and ERK2 (p44 and p42 MAP Kinase). ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The downstream effects of ERK1/2 signaling pathway are linked to the regulation of cell growth and differentiation as well as the cytoskeleton. Downstream targets of ERK1/2 contain p90RSK and the transcription factor Elk-1. ERK1 and ERK2 are phosphorylated within the activation loop on both a threonine and a tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1/2. ERK1/2 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases.

GenScript Rabbit Anti-ERK2 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide (KRIEVEQALAHPYLEQYYDPSDE) corresponding to 300-322 residues of human ERK2 (Swiss Prot: P28482).

Synonyms

Rabbit Anti-ERK2 pAb;

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Citations

1. Schwartz Kimberly, et al. Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. Electrophoresis. (2017)