Prohibitin Antibody, pAb, Rabbit
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Prohibitin Antibody, pAb, Rabbit  |
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| Full Name |
Prohibitin Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti Prohibitin Polyclonal Antibody; |
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Description |
Prohibitin is believed to be a negative regulator of cell proliferation and may also act as a tumor suppressor. Mutations in prohibitin have been linked to sporadic breast cancer. Prohibitin inhibits DNA synthesis and plays a role in proliferation regulation. As yet, it is unclear if it is the protein or the mRNA that exhibits these effects. Prohibitin may play a role in regulating mitochondrial respiration activity and aging. Prohibitin is located at the inner mitochondrial membrane and is widely expressed in various tissues.
GenScript Rabbit Anti-Prohibitin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human prohibitin protein (Swiss Prot: P35232). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Prohibitin Polyclonal Antibody detects endogenous levels of human prohibitin protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) derived from within residues 50-100 of human prohibitin (Swiss Prot: P35232) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
Predicted Band Size |
30 kD |
Observed Band Size |
30 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 8680_20081127022030.PDF (PDF)