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DJ-1 Antibody, pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti DJ-1 Polyclonal Antibody

Documents
MSDS: 20081221224712 (PDF)
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Reference

Description

DJ-1 belongs to the C56 peptidase family. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone and a sensor for oxidative stress. It apparently protects neurons against oxidative stress and cell death. Defects in PARK7 gene cause autosomal recessive early-onset Parkinson disease 7. Specifically, defects in PARK7 are characterized by PD onset before the age of 40, slow progression of the condition, and initial good response to levodopa. Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. However, cases of early-onset PD are sometimes found. PD generally arises as a sporadic condition but can be inherited as a simple Mendelian trait. Although sporadic and familial PD are very similar, inherited forms of the disease usually begin at an earlier age and are associated with atypical clinical features.
PD is characterized by bradykinesia, resting tremors, muscular rigidity, and postural instability as well as by a significant clinical response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of protein aggregates) in surviving neurons at various areas of the brain.
GenScript Rabbit Anti-DJ-1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 50-100 of human DJ-1 protein (Swiss Prot: Q99497).

Ig Subclass

Rabbit IgG

Storage

GenScript Rabbit Anti-DJ-1 Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Specificity

GenScript Rabbit Anti-DJ-1 Polyclonal Antibody detects endogenous levels of human and mouse DJ-1 protein. Sequence homology predicts that it will also react with rat DJ-1 protein

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

Synthetic peptide (KLH-coupled) within residues 50-100 of human DJ-1 protein (Swiss Prot: Q99497)

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

20 kD

Observed Band Size

24 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
IHC: 5-10 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse. This product has not yet been tested with other species.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.


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