AP2M1 Antibody, pAb, Rabbit
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AP2M1 Antibody, pAb, Rabbit  |
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| Full Name |
AP2M1 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-AP2M1 pAb; |
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Description |
AP2M1 is a subunit of heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complex medium subunit family. AP2 is composed of two large adaptins (alpha-type subunit AP2A1, or AP2A2, and beta-type subunit AP2B1), one medium adaptin (mu-type subunit AP2M1), and a small adaptin (sigma-type subunit AP2S1). The AP2 complex is part of the protein coat linking clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins so that they can be selected and concentrated.
GenScript Rabbit Anti-AP2M1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human AP2M1 protein (Swiss Prot: Q96CW1). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-AP2M1 Polyclonal Antibody detects human AP2M1 protein. Sequence homology predicts that it will also react with rat and mouse AP2M1 protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Synthetic peptide (KLH-coupled) within residues 150-200 of human AP2M1 protein (Swiss Prot: Q96CW1) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
50 kD |
Observed Band Size |
50 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become trapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 9070_20081228203925.PDF (PDF)