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Parkin Antibody, pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-Parkin pAb

Documents
MSDS: 20090121041527 (PDF)
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Reference

Description

Parkin, also known as PARKIN2, is a novel protein in Ubl conjugation pathway. Parkin functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include CCNE1, SYT11, GPR37, and STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. Parkin may play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. The protein may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. Defects in PARK2 are a cause of Parkinson disease (PD).
GenScript Parkin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Parkin Antibody, pAb, Rabbit detects endogenous levels of human, mouse and rat parkin protein.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260)

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder (40µg) with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

51 kD

Observed Band Size

51 kD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human, mouse, and rat. This product has not yet been tested with other species.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)


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