Parkin Antibody, pAb, Rabbit
|
Parkin Antibody, pAb, Rabbit  |
|
|
| Full Name |
Parkin Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-Parkin pAb |
|
Description |
Parkin, also known as PARKIN2, is a novel protein in Ubl conjugation pathway. Parkin functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include CCNE1, SYT11, GPR37, and STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. Parkin may play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. The protein may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. Defects in PARK2 are a cause of Parkinson disease (PD).
GenScript Parkin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260). |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Parkin Antibody, pAb, Rabbit should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
GenScript Parkin Antibody, pAb, Rabbit detects endogenous levels of human, mouse and rat parkin protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide within residues 300-350 of human parkin protein (Swiss Prot: O60260) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder (40µg) with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
51 kD |
Observed Band Size |
51 kD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. | |
MSDS: 20090121041527 (PDF)
