Calbindin Antibody, pAb, Rabbit
|
Calbindin Antibody, pAb, Rabbit  |
|
|
| Full Name |
Calbindin Antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-Calbindin Polyclonal Antibody |
|
Description |
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27 kD protein induced by vitamin D in the duodenums of young birds. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains four active calcium-binding domains and two modified domains that presumably have lost their calcium-binding capacity. The neurons in the brains of patients with Huntingtons disease are calbindin-depleted.
GenScript Rabbit Anti-Calbindin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from residues 150-200 of human calbindin protein (Swiss Prot: P05937). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Calbindin Polyclonal Antibody detects human calbindin protein. Sequence homology predicts that it will also to react with mouse and rat calbindin. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from residues 150-200 of human calbindin protein (Swiss Prot: P05937) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
IHC: 5-10 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Human. This product has not yet been tested with other species. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
MSDS: 20090131205137 (PDF)
