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TF-tag Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01329-200	TF-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01329-40	TF-tag Antibody, pAb, Rabbit	40 μg	$50

Full Name

TF-tag Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-TF-tag pAb;

Documents
Document-MSDS: 9586_20090413220518.PDF (PDF)
Figures
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Reference

Description

Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems.
Trigger Factor (TF) is a prokaryotic ribosome-associated chaperone protein (48 kDa) which facilitates co-translational folding of newly expressed proteins, thus enabling efficient soluble protein production for otherwise intractable target proteins.

GenScript Rabbit Anti-TF-tag polyclonal Antibody is developed in rabbit using purified recombinant TF protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-TF-tag polyclonal antibody specifically reacts with fusion proteins containing TF tag. The antibody can also detect endogenous levels of E.coli trigger factor.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

Purified recombinant TF protein

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other Applications: user-optimized

Conjugation

Unconjugated

Host Species

Rabbit

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

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