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Neurofascin Antibody, pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-Neurofascin pAb

Documents
MSDS: 20090420033947 (PDF)
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Description

Neurofascin is one member of Li family which involves vertebrate L1, Ng-CAM, and Nr-CAM. Neurofascin protein is a nervous system cell adhesion molecule that contains six Ig domains and multiple fibronectin type III repeats in its extracellular region. Expression level of neurofascin is abundant in the nervous system during early development as well as in adult stage. Phosphorylation of the FIGQY tyrosine of neurofascin abolishes ankyrin binding and increases lateral mobility of neurofascin. Phospho-FIGQY neurofascin associates with doublecortin, which probably plays an important role in neuronal migration.

GenScript Rabbit Anti-Neurofascin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from amino terminal of human Neurofascin. (Swiss Prot: O94856).

Ig Subclass

Rabbit IgG

Storage

GenScript Rabbit Anti-Neurofascin Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at -20°C or below. The reconstituted antibody can be stored for two to three weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.

Specificity

GenScript Rabbit Anti-Neurofascin Polyclonal Antibody detects endogenous levels of rat and mouse Neurofascin protein. It is predicted to react with human neurofascin protein according to sequence homology.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide from amino terminal of human Neurofascin (Swiss Prot: O94856).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with 80 µl of deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 1-2 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Rat and mouse. This product has not been tested with other species yet.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.


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