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COX IV Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01402-200	COX IV Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01402-40	COX IV Antibody, pAb, Rabbit	40 μg	$50

Full Name

COX IV Antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-COX IV pAb;

Documents
Document-MSDS: 9776_20090517233748.PDF (PDF)
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Reference

Description

Cytochrome c oxidase (COX) is a terminal enzyme complex involved in the mitochondrial electron transport chain. It consists of 13 subunits and is localized at the inner mitochondrial membrane. One of the small subunits of COX IV is encoded by nuclear DNA while the 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA. Deficiency in COX activity leads to a number of human diseases. The COX IV antibody can be used effectively as a mitochondrial loading control.

GenScript Rabbit Anti-COX IV Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human COX IV. (Swiss Prot: P13073).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-COX IV Polyclonal Antibody detects endogenous levels of human and mouse COX IV protein. It is predicted to react with rat according to sequence homology.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Immunogen

KLH-coupled synthetic peptide from C-terminal of human COX IV (Swiss Prot: P13073).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human and mouse. This product has not yet been tested with other species.

Host Species

Rabbit

Antigen Species

Human

Note

Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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