CDK5 Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A01438-40
40 μg
$79
CDK5 Antibody, pAb, Rabbit
Full Name CDK5 Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-Cyclin dependent kinase 5 antibody;
Documents
CDK5 Antibody, pAb, RabbitDocument-MSDS: 10073_20090702054010.PDF (PDF)
Figures
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Description
Cyclin dependent kinase 5 (CDK5), a member of the cyclin-dependent kinase family, plays crucial roles in neuronal differentiation, migration, axon outgrowth and synaptogenesis. CDK5 is a proline-directed serine/threonine (Ser/Thr) kinase that phosphorylates a diverse range of substrates, such as histone H1, TAU, MAP2 and NF-H and NF-M. The activity of CDK5 depends on the combination of neuron specific activator proteins, p35 or p39 or their truncated forms, p25 and p29. CDK5 phosphorylates the transcription factor myocyte enhancer factor 2 in mediation of neurotoxic effects. The conversion of p35 to p25 by calpain gives rise to prolonged activation of CDK5. Deregulated CDK5 activity has been implicated in neurodegenerative diseases, including Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis.

GenScript Rabbit Anti-CDK5 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human CDK5 (Swiss Prot: Q00535).
Ig Subclass
Rabbit IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Rabbit Anti-CDK5 Polyclonal Antibody detects endogenous levels of human, mouse, and rat CDK5.
Positive Control: Hela, HEK293, mouse brain and rat brain.
Formulation
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen
KLH-coupled synthetic peptide from C-terminal of human CDK5 (Swiss Prot: Q00535).
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human, mouse, and rat. This product has not been tested for other species yet.
Host Species
Rabbit
Antigen Species
Human
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
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Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
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Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.