SNAP25 Antibody, pAb, Rabbit
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SNAP25 Antibody, pAb, Rabbit  |
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| Full Name |
SNAP25 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Synaptosomal Associated Protein 25 antibody; |
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Description |
SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that is involved in the molecular regulation of neurotransmitter release. It is part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. SNAP25 plays an important role toward achieving synaptic function of specific neuronal systems and it associates with proteins involved in vesicle docking and membrane fusion. In addition, SNAP25 plays an essential role in synaptic vesicle fusion and exocytosis.
GenScript Rabbit Anti-SNAP25 Polyclonal Antibody is developed in rabbit using Purified His-tagged human SNAP25 (Swiss Prot: Q5U0B5) full length recombinant protein.
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-SNAP25 Polyclonal Antibody detects endogenous levels of human, mouse, and rat SNAP25.
Positive Control: Hela, SH-SY5Y, mouse brain and rat brain.
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Purified His-tagged human SNAP25 (Swiss Prot: Q5U0B5) full length recombinant protein. |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Immunoprecipitation: 2-10 µg/mg of lysate
Other Applications: user-optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not been tested for other species yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 10101_20090709082542.PDF (PDF)