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Creatine kinase MB Antibody, pAb, Rabbit  |
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| Full Name |
Creatine kinase MB Antibody, pAb, Rabbit
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Abbreviated name-1 |
CKMB antibody; |
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Description |
Creatine kinase (CK) is a major enzyme involved in energy metabolism in tissues with large fluctuations of energetic demand. CK catalyses the reversible transfer of a high energy phosphoryl group between ATP and phosphocreatine. Creatine kinase consists of B subunit (brain type) and M subunit (muscle type) and therefore three different cytosolic isoenzymes exist: CK-MM, CK-BB and CK-MB. The major muscle isoform, CK-MM, is a homodimer of the M subunit. In contrast to muscle, the major creatine kinase isoform in brain is BB creatine kinase. In a few tissues, especially myocardium, a heterodimeric isoform MB creatine kinase is present.
GenScript Rabbit Anti-Creatine kinase MB Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 300~350 of human Creatine kinase M (Swiss Prot: P06732) and Creatine kinase B (Swiss Prot: P12277).
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-Creatine kinase MB Polyclonal Antibody detects endogenous levels of human, mouse and rat Creatine kinase M and Creatine kinase B.
Positive Control: HEK293, SH-SY5Y, mouse brain and rat brain.
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide within residues 300-350 of human Creatine kinase M (Swiss Prot: P06732) and Creatine kinase B (Swiss Prot: P12277) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user-optimized
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Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, and rat. This product has not been tested for other species yets. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 10236_20090723065256.PDF (PDF)