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Annexin II Antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01471-200	Annexin II Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01471-40	Annexin II Antibody, pAb, Rabbit	40 μg	$50

Full Name

Annexin II Antibody, pAb, Rabbit

Abbreviated name-1

Annexin A2 antibody;

Documents
Document-MSDS: 10330_20090812081024.PDF (PDF)
Figures
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Reference

Description

Annexin II is one member of the annexin family of calcium-dependent phospholipids binding proteins. It plays a role in the regulation of membrane and actin dynamics particularly in the endocytic pathway. A tyrosine phosphorylation switch in Annexin II triggers Rho/ROCK- dependent and actin-mediated changes in cell morphology associated with the control of cell adhesion. It may play roles in p53 induced apoptosis and it is also involved in regulation of cell proliferation. Annexin II is binding partner of SHP2 and the binding of SHP2 with Annexin II is affected by EGF stimulation, extracellular calcium levels, and the levels of HSP70.
GenScript Rabbit Anti-Annexin II Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 180-230 of human Annexin II protein (Swiss Prot: P07355).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-Annexin II Polyclonal Antibody detects endogenous levels of human Annexin II. Predicted to react with rat and mouse Annexin II according to sequence homology.
Positive Control: Hela and HepG2.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

KLH-coupled synthetic peptide within residues 180-230 of human Annexin II protein (Swiss Prot: P07355).

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Conjugation

Unconjugated

Species Reactivity

Human. Reactivity to other species is not tested yet.

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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