| Full Name |
H1N1 HA antibody, pAb, Rabbit
|
Abbreviated name-1 |
Rabbit Anti-H1N1 HA polyclonal antibody; Hemagglutinin of Influenza A Virus antibody |
|
Description |
Influenza A (H1N1) virus is a subtype of influenzavirus A and the most common cause of influenza (flu) in humans. A strain of swine-origin H1N1 was responsible for the 2009 flu pandemic. HA (Hemagglutinin) is an antigenic glycoprotein found on the surface of the H1N1 virus. And it is responsible for binding the virus to the cell that is being infected.GenScript Rabbit Anti-H1N1 HA Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within 350~400 of Influenza A (H1N1) 2009 virus HA protein (GenBank: CY039999). |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Rabbit Anti-H1N1 HA Polyclonal Antibody detects influenza A (H1N1) HA protein. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
KLH-coupled synthetic peptide within 350~400 of Influenza A (H1N1) 2009 virus HA protein (GenBank: CY039999). |
Purification Method |
Immunoaffinity chromatography. |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
115 KD |
Observed Band Size |
115 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml.
Other Applications: user optimized.
|
Conjugation |
Unconjugated |
Species Reactivity |
Influenza A virus (H1N1) |
Host Species |
Rabbit |
Antigen Species |
Influenza A |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
Document-MSDS: 11090_20091023055836.PDF (PDF)
