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NQO1 antibody, pAb, Rabbit

Cat. No.	Name	Size	Price
A01511-200	NQO1 antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A01511-40	NQO1 antibody, pAb, Rabbit	40 μg	$50

Full Name

NQO1 antibody, pAb, Rabbit

Abbreviated name-1

Rabbit Anti-NQO1 polyclonal antibody; NQO1 antibody

Documents
Document-MSDS: 11138_20091027034425.PDF (PDF)
Figures
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Reference

Description

Quinone oxidoreductase 1 (NQO1) is a cytosolic flavoenzyme that catalyzes the two-electron reduction of a broad range of substrates. It is one member of the NAD(P)H dehydrogenase (quinone) family. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. NQO1 can serve as a detoxification enzyme for it has the ability to reduce quinone substrates directly to their less toxic hydroquinone derivatives. NQO1 can also function as an antioxidant enzyme reducing ubiquinone and vitamin E quinone to their antioxidant forms. GenScript Rabbit Anti-NQO1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human NQO1 (Swiss Prot: P15559).

Ig Subclass

Rabbit IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Rabbit Anti-NQO1 Polyclonal Antibody detects endogenous levels of human NQO1.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

KLH-coupled synthetic peptide from C-terminal of human NQO1 (Swiss Prot: P15559).

Purification Method

Immunoaffinity chromatography.

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Predicted Band Size

31 KD

Observed Band Size

31 KD

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other Applications: user optimized

Conjugation

Unconjugated

Species Reactivity

Human

Host Species

Rabbit

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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