HS1 Antibody, pAb, Rabbit
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HS1 Antibody, pAb, Rabbit  |
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| Full Name |
HS1 Antibody, pAb, Rabbit
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Abbreviated name-1 |
p75 antibody, pAb, Rabbit; |
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Description |
HS1 also known as HCLS1, is a substrate of the antigen receptor-coupled tyrosine kinase. The phosphorylation of HS1 catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets. HS1 has been implicated in the signal transduction pathways in B and T lymphocytes. It plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. It may also be involved in the regulation of gene expression.
GenScript HS1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 250-300 of human HS1 (Swiss Prot: P14317).
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript HS1 Antibody, pAb, Rabbit detects endogenous levels of human HS1.
Positive Control: Jurkat
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide within residues 250-300 of human HS1 (Swiss Prot: P14317) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
40 KD |
Observed Band Size |
75 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user optimized.
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Conjugation |
Unconjugated |
Species Reactivity |
Human. Reactivity to other species is not tested yet. |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 12349_20100330025831.PDF (PDF)