cPLA2 Antibody, pAb, Rabbit
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cPLA2 Antibody, pAb, Rabbit  |
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| Full Name |
cPLA2 Antibody, pAb, Rabbit
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Abbreviated name-1 |
Cytosolic phospholipase A2 antibody, pAb, Rabbit; |
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Description |
Cytosolic phospholipase A2 (cPLA2) catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in mitogenesis, differentiation, inflammation, and cytotoxicity. Its catalytic activity is stimulated via phosphorylation of cPLA2 at Ser505 and Ser727. cPLA2 is expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts, and lung endothelium.
GenScript cPLA2 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human cPLA2 (Swiss Prot: P47712).
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Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript cPLA2 Antibody, pAb, Rabbit detects endogenous levels of mouse cPLA2. It is predicted to react with human and rat cPLA2 according to sequence homology.
Positive Control: Mouse heart
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Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
KLH-coupled synthetic peptide from C-terminal of human cPLA2 (Swiss Prot: P47712) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Predicted Band Size |
85 KD |
Observed Band Size |
65 KD |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse |
Host Species |
Rabbit |
Antigen Species |
Human |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 12316_20100323010903.PDF (PDF)