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THETM BrdU Antibody, mAb, Mouse


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Cat. No.
Product Name
Size
Price
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A01650-100 THETM BrdU Antibody, mAb, Mouse
100 μg
$157.00



Abbreviated name-1 Bromodeoxyuridine Antibody, mAb, Mouse; Bromo-deoxyuridine Antibody, mAb, Mouse
Description Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.
GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
Immunogen BrdU conjugated to KLH
Host Species Mouse
Conjugation Unconjugated
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Method Protein A affinity column

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Clone ID 3E2D3
Ig Subclass IgG1, κ
Specificity The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T).
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.015- 0.05 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse
THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

Flow cytometric analysis of untreated or BrdU incorporated Hela cells using GenScript THETM BrdU Antibody, mAb, Mouse (yellow
and cyan, respectively), Competitor A Mouse Anti-BrdU mAb (blue and purple, respectively), or mouse IgG1 isotype control (green and red, respectively).

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse
THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of Hela cells incorporated with BrdU using THETM BrdU Antibody, mAb, Mouse (GenScript, A01650)

The image was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse
THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

Competitive ELISA analysis of Cross Reactivity using THETM BrdU Antibody, mAb, Mouse (GenScript, A01650)

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse
THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

Comparison of THETM BrdU Antibody, mAb, Mouse (GenScirpt, A01650) with Mouse Anti-BrdU mAb (Competitor A) by indirect ELISA.

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse

THE<sup>TM</sup> BrdU Antibody, MAb, Mouse


Cat. No.
Product Name
Size
Price
Quantity
A01653 THETM BrdU Antibody [HRP], mAb, Mouse
100 μg
$165.00


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