Catalog Products » Antibodies » THE™ Elite Antibodies » THETM BrdU Antibody, mAb, Mouse
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Name
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A01650-100
100 μg
$157
THETM BrdU Antibody, mAb, Mousecustom antibody production
THE<sup>TM</sup> BrdU Antibody, mAb, Mouse
Full Name THETM BrdU Antibody, mAb, Mouse
Abbreviated name-1
Bromodeoxyuridine Antibody, mAb, Mouse; Bromo-deoxyuridine Antibody, mAb, Mouse
Documents
THE<sup>TM</sup> BrdU Antibody, mAb, MouseDocument-MSDS: 12765_20100514045629.PDF (PDF)
THE<sup>TM</sup> BrdU Antibody, mAb, MouseDocument-COA: 10C000080_A01650_COA.pdf (pdf)
Figures
THE<sup>TM</sup> BrdU Antibody, mAb, Mouse zoomTHE<sup>TM</sup> BrdU Antibody, mAb, Mouse
THE<sup>TM</sup> BrdU Antibody, mAb, Mouse zoomTHE<sup>TM</sup> BrdU Antibody, mAb, Mouse
THE<sup>TM</sup> BrdU Antibody, mAb, Mouse zoomTHE<sup>TM</sup> BrdU Antibody, mAb, Mouse
THE<sup>TM</sup> BrdU Antibody, mAb, Mouse zoomTHE<sup>TM</sup> BrdU Antibody, mAb, Mouse
Citations
Description
Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.
GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
Ig Subclass
IgG1, κ
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T).
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
BrdU conjugated to KLH
Clone ID
3E2D3
Purification Method
Protein A affinity column
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.015- 0.05 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other applications: user-optimized
Fusion Partner
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Conjugation
Unconjugated
Host Species
Mouse
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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