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THE TM BrdU Antibody, mAb, Mouse  |
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| Full Name |
THETM BrdU Antibody, mAb, Mouse
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Abbreviated name-1 |
Bromodeoxyuridine Antibody, mAb, Mouse; Bromo-deoxyuridine Antibody, mAb, Mouse |
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Description |
Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange.
GenScript THETM BrdU Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH. |
Ig Subclass |
IgG1, κ |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2'-deoxyuridine (BrdU), 5-chloro-2'-deoxyuridine (CldU) and 5-iodo-2'-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2'-deoxyuridine (FdU) and thymidine (T). |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
BrdU conjugated to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.015- 0.05 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
Other applications: user-optimized
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Fusion Partner |
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 12765_20100514045629.PDF (PDF)
