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FGF-basic Antibody (3B10F9), mAb, Mouse

Cat. No.	Name	Size	Price
A01718-200	FGF-basic Antibody (3B10F9), mAb, Mouse	200 μg(5x40μg)	$225
A01718-40	FGF-basic Antibody (3B10F9), mAb, Mouse	40 μg	$50

Full Name

FGF-basic Antibody (3B10F9), mAb, Mouse

Abbreviated name-1

Basic fibroblast growth factor antibody;

Documents
Document-MSDS: 13560_20101227004102.PDF (PDF)
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Reference

Description

FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. It is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. They are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
GenScript FGF-basic Antibody (3B10F9), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant FGF-basic protein (Swiss-Prot: P09038).

Ig Subclass

IgG2a,k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript FGF-basic Antibody (3B10F9), mAb, Mouse detects human FGF-basic protein and does not react with the other human cytokines such as FGF-6, FGF-16, FGF-17and FGF-acidic.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Immunogen

human recombinant FGF-basic protein (Swiss-Prot: P09038).

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/ml
ELISA Detection: 0.05-0.2 µg/ml
Other Applications: user-optimized

Fusion Partner

Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.

Conjugation

Unconjugated

Species Reactivity

Human. Reactivity to other species is not tested yet.

Host Species

Mouse

Antigen Species

Human

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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