Catalog Products » beta Actin Antibody, mAb, Rabbit
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beta Actin Antibody, mAb, Rabbit

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells.Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction.
$102.00
A02366-50

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Overview
Specificity This product is specific to beta Actin
Host Species Rabbit
Immunogen A synthesized peptide derived from human beta Actin
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:10000-1:20000
Immunohistochemistry (IHC) 1:100-1:500
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:500
Immunohistochemistry Frozen (IHF) 1:100-1:500
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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Beta Actin Antibody, MAb, Rabbit

Western blot analysis of beta Actin expression in (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate. »

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Background
Target Background Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells.Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction.
Synonyms ACTIN; ACTB; BETA-ACTIN; beta actin; actin beta;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.