p-CDK1/2 (T14) Antibody, mAb, Rabbit

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase.

Price	$210.00
Cat. No.	A02871-50
Size	50 μl 100 μl Buy in bulk
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	This product is specific to p-CDK1/2 (T14)
Host Species	Rabbit
Immunogen	A synthesized peptide derived from human CDK1 around the phosphorylation site of T14
Species Reactivity	Human; Mouse; Rat
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1000-1:2000
Immunohistochemistry (IHC)	1:100-1:200
Immunoprecipitation (IP)	1:20-1:50

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Properties

Form	Liquid
Storage Buffer	Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal

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Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea. »

Background

Target Background	Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase.
Synonyms	CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase;

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.