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Manual
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HES1 Antibody, mAb, Rabbit

HES1 is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors which function primarily to repress transcription of bHLH-dependent genes. HES1 is understood to play an important conserved role in maintaining pluripotency of embryonic and adult stem/progenitor cells via the transcriptional repression of genes that promote differentiation.
$210.00
A03032-50

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Overview
Specificity This product is specific to HES1
Host Species Rabbit
Immunogen A synthesized peptide derived from human HES1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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HES1 Antibody, MAb, Rabbit

Western blot analysis of Hes1 expression in SH-SY5Y cell lysate. »

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Background
Target Background HES1 is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors which function primarily to repress transcription of bHLH-dependent genes. HES1 is understood to play an important conserved role in maintaining pluripotency of embryonic and adult stem/progenitor cells via the transcriptional repression of genes that promote differentiation.
Synonyms HES1; BHLHb39; HHL; HL; Hairy and enhancer of split 1; Hairy homolog; HRY; Hairy homolog (Drosophila); Transcription factor HES-1; Hairy-like protein; HES-1;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.