Catalog Products » p-STAT1 (S727) Antibody, mAb, Rabbit
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p-STAT1 (S727) Antibody, mAb, Rabbit

Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus.
$210.00
A03085-50

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Overview
Specificity This product is specific to p-STAT1 (S727)
Host Species Rabbit
Immunogen A synthesized peptide derived from human STAT1 around the phosphorylation site of S727
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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P-STAT1 (S727) Antibody, MAb, Rabbit

Western blot analysis of Phospho-STAT1 (S727) expression in HeLa cell lysate treated with etoposide. »

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Background
Target Background Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus.
Synonyms Signal transducer and activator of transcription 1; Transcription factor ISGF-3 components p91/p84; STAT1; CANDF7; ISGF3;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.