Catalog Products » p-YAP1 (S127) Antibody, mAb, Rabbit
Manual
Manual
COAs

p-YAP1 (S127) Antibody, mAb, Rabbit

Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis.The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ.
$210.00
A03126-50

Ask us a question
Overview
Specificity This product is specific to p-YAP1 (S127)
Host Species Rabbit
Immunogen A synthesized peptide derived from human YAP1 around the phosphorylation site of S127
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
Back

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:5000
Immunohistochemistry (IHC) 1:100-1:200
Back

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Back

P-YAP1 (S127) Antibody, MAb, Rabbit

Western blot analysis of Phospho-YAP1 (S127) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with FBS+calyculin A. »

<
>

Background
Target Background Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis.The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ.
Synonyms 65 kDa Yes associated protein; YAP; YAp 1; YAP2; YAP 65;YKI; Yorkie homolog;
Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.