Catalog Products » p-PKC zeta (T560) Antibody, mAb, Rabbit
Manual
Manual
COAs

p-PKC zeta (T560) Antibody, mAb, Rabbit

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation.
$210.00
A03169-50

Ask us a question
Overview
Specificity This product is specific to p-PKC zeta (T560)
Host Species Rabbit
Immunogen A synthesized peptide derived from human PKC zeta around the phosphorylation site of T560
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
Back

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Back

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Back

P-PKC Zeta (T560) Antibody, MAb, Rabbit

Western blot analysis of Phospho-PKC zeta (T560) expression in HeLa cell treated with Calyculin A lysate. »

<
>

Background
Target Background Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation.
Synonyms aPKCzeta; nPKC zeta; PKC 2; PKC ZETA; PKCZETA ; Protein kinase C zeta; r14-3-3;
Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.