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ATF5 Antibody, mAb, Rabbit

Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate.Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation.
$210.00
A03260-50

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Overview
Specificity This product is specific to ATF5
Host Species Rabbit
Immunogen A synthesized peptide derived from human ATF5
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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ATF5 Antibody, MAb, Rabbit

Western blot analysis of ATF5 expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) C6 cell lysate. »

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Background
Target Background Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate.Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation.
Synonyms Cyclic AMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; ATF5; ATFX; NAP1; NRIF3 associated protein; ODA 10;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.