p-Hsp27 (S78) Antibody, mAb, Rabbit

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization.

Price	$210.00
Cat. No.	A03339-50
Size	50 μl 100 μl Buy in bulk
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	This product is specific to p-Hsp27 (S78)
Host Species	Rabbit
Immunogen	A synthesized peptide derived from human Hsp27 around the phosphorylation site of S78
Species Reactivity	Human
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1000-1:2000
Immunohistochemistry (IHC)	1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF)	1:50-1:200
Immunohistochemistry Frozen (IHF)	1:50-1:200
Immunoprecipitation (IP)	1:20-1:50

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Properties

Form	Liquid
Storage Buffer	Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal

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Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin. »

Background

Target Background	Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization.
Synonyms	28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27;

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.