p-Histone H2A.X (S139) Antibody, mAb, Rabbit

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Price	$210.00
Cat. No.	A03385-50
Size	50 μl 100 μl Buy in bulk
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	This product is specific to p-Histone H2A.X (S139)
Host Species	Rabbit
Immunogen	A synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139
Species Reactivity	Human; Mouse; Rat
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1000-1:5000
Immunohistochemistry (IHC)	1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF)	1:50-1:200
Immunohistochemistry Frozen (IHF)	1:50-1:200
Immunoprecipitation (IP)	1:20-1:50

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Properties

Form	Liquid
Storage Buffer	Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal

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All lanes use the p-Histone H2A.X (S139) Antibody, mAb, Rabbit at 1:500 dilution for 1 hour at room temperature. »

Background

Target Background	Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Synonyms	H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X;

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.