Catalog Products » K63-linkage specific ubiquitin Antibody, mAb, Rabbit
Manual
Manual
COAs

K63-linkage specific ubiquitin Antibody, mAb, Rabbit

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).
$210.00
A03392-50

Ask us a question
Overview
Specificity This product is specific to K63-linkage specific ubiquitin
Host Species Rabbit
Immunogen A synthesized peptide derived from human K63-linkage specific ubiquitin
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
Back

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100
Back

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Back

K63-linkage Specific Ubiquitin Antibody, MAb, Rabbit

Western blot analysis of Ubiquitin expression in K63-linked-Ub2 recombinant protein. »

<
>

Background
Target Background Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains).
Synonyms FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.