Catalog Products » PI3 kinase p85 alpha Antibody, mAb, Rabbit
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PI3 kinase p85 alpha Antibody, mAb, Rabbit

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
$210.00
A03724-50

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Overview
Specificity This product is specific to PI3 kinase p85 alpha
Host Species Rabbit
Immunogen A synthesized peptide derived from human PI3 kinase p85 alpha
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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PI3 Kinase P85 Alpha Antibody, MAb, Rabbit

Western blot analysis of PI3 Kinase p85 alpha expression in (1) K562 cell lysate; (2) Raw 264.7 cell lysate; (3) C6 cell lysate. »

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Background
Target Background Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
Synonyms GRB1; p85 alpha; PI3-kinase subunit p85-alpha; PI3K; PI3K regulatory subunit alpha; Pik3r1; PtdIns 3 kinase p85 alpha;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.