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KDM1B Antibody, mAb, Rabbit

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis.
$210.00
A03902-50

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Overview
Specificity This product is specific to KDM1B
Host Species Rabbit
Immunogen A synthesized peptide derived from human KDM1B
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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KDM1B Antibody, MAb, Rabbit

Western blot analysis of LSD2 / AOF1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC12 cell lysate. »

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Background
Target Background Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis.
Synonyms AOF1; KDM1B; LSD2; Lysine-specific histone demethylase 1B; Lysine-specific histone demethylase 2;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.