Catalog Products » p-MLKL (S358) Antibody, mAb, Rabbit
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p-MLKL (S358) Antibody, mAb, Rabbit

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.
$210.00
A03953-50

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Overview
Specificity This product is specific to p-MLKL (S358)
Host Species Rabbit
Immunogen A synthesized peptide derived from human MLKL around the phosphorylation site of S358
Species Reactivity Human
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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P-MLKL (S358) Antibody, MAb, Rabbit

Western blot analysis of Phospho-MLKL (S358) expression in (1) HT-29 cell lysate; (2) HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD. »

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Background
Target Background Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.
Synonyms hMLKL; Mlkl;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.