Sestrin 1 Antibody, mAb, Rabbit

Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway.

Price	$210.00
Cat. No.	A04515-50
Size	50 μl 100 μl Buy in bulk
Quantity
Est. Delivery


Ask us a question

Overview Applications Properties Background Bottom Notes

Overview

Specificity	This product is specific to Sestrin 1
Host Species	Rabbit
Immunogen	A synthesized peptide derived from human Sestrin 1
Species Reactivity	Human; Mouse; Rat
Conjugate	Unconjugated

Back

Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1000-1:2000
Flow Cytometry	1:20-1:100

Back

Properties

Form	Liquid
Storage Buffer	Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal

Back

Western blot analysis of SESN1 expression in K562 cell lysate. »

Background

Target Background	Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway.
Synonyms	PA26; sesn1; SEST1; sestrin 1;

Back

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.