Rab27A Antibody, mAb, Rabbit

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Price	$210.00
Cat. No.	A04559-50
Size	50 μl 100 μl Buy in bulk
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Overview Applications Properties Background Bottom Notes

Overview

Specificity	This product is specific to Rab27A
Host Species	Rabbit
Immunogen	A synthesized peptide derived from human Rab27A
Species Reactivity	Human
Conjugate	Unconjugated

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Applications

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF)	1:50-1:200
Immunoprecipitation (IP)	1:20-1:50
Flow Cytometry	1:20-1:100

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Properties

Form	Liquid
Storage Buffer	Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Affinity-chromatography
Isotype	Rabbit IgG
Clonality	Monoclonal

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Western blot analysis of RAB27A expression in MCF-7 cell lysate. »

Background

Target Background	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Synonyms	GS2; RAM; RAB27; HsT18676;

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.