Catalog Products » Cytochrome P450 1A2 Antibody, mAb, Rabbit
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Cytochrome P450 1A2 Antibody, mAb, Rabbit

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase).
$210.00
A04745-50

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Overview
Specificity This product is specific to Cytochrome P450 1A2
Host Species Rabbit
Immunogen A synthesized peptide derived from human Cytochrome P450 1A2
Species Reactivity Human
Conjugate Unconjugated
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Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Flow Cytometry 1:20-1:100
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Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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Cytochrome P450 1A2 Antibody, MAb, Rabbit

Western blot analysis of Cytochrome P450 1A2 expression in Caco2 cell lysate. »

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Background
Target Background A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase).
Synonyms CP12; CYP1A2; CYPIA2; P3 450; P450 4; P450 P3;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.