Catalog Products » THETM 25-OH Vitamin D3 Antibody (32F9C4), mAb, Mouse
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25-OH VD3 (32F9C4), mAb, Mouse

Vitamin D plays an important role in calcium homeostasis and metabolism. Vitamin D3 and vitamin D2 are two forms of vitamin D in human body, both of which only a few foods contain. The deficiency of vitamin D in human body can cause a threat of diseases such as osteomalacia, rickets and diabetes. 25-OH Vitamin D2 and 25-OH Vitamin D3 as vitamin D metabolites are measured in human serum to evaluate vitamin D level. Vitamin D supplements are considered as an effective way to treat or to prevent the diseases.
$219.00
V00101-0.1

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Overview
Specificity Mab 32F9C4 recognizes 25-OH Vitamin D3 and has no cross-reactivity with 25-OH Vitamin D2.
The specificity of the antibody is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system.
Compound Cross-reactivity (%)
25-OH VD3 100
1α, 25-OH VD3 78
24R, 25-2OH VD3 36
25-OH VD2 0.058
1α, 25-(OH)2 VD2 0.094
VD2 0.004
VD3 0.026
Clone ID 32F9C4
Isotype IgG2a
Subclass IgG2a
Host Species Mouse
Antigen Species Human
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Properties
Conjugation Unconjugated
Production Cultured in vitro under conditions free from animal-derived components
Purification Protein A/G affinity column
Concentration ≥ 2mg/ml
Formulation 50 mM Na-citrate, 150 mM NaCl, pH 7.0, containing 0.03% ProClin 300.
Storage For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Product Stability
Temperature, Time Results for clone 32F9C4
-80°C, 21 days OK
-20°C, 21 days OK
4°C, 21 days OK
20°C, 21 days OK
37°C, 21 days OK
40°C, 21 days OK
Note Mab 32F9C4 can be used with 25-OH Vitamin D3-BSA Conjugate (GenScript, T00101) or 25-OH Vitamin D3-HRP Conjugate (GenScript, T00102) to compete with 25-OH Vitamin D3 in competitive immunoassay.
GenScript can customize this product per customer's request including product size, buffer components, etc.
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Applications
ELISA, CLIA, GICA, LF, and LTIA
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25-OH VD3 (32F9C4), MAb, Mouse

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Background
Target Background Vitamin D plays an important role in calcium homeostasis and metabolism. Vitamin D3 and vitamin D2 are two forms of vitamin D in human body, both of which only a few foods contain. The deficiency of vitamin D in human body can cause a threat of diseases such as osteomalacia, rickets and diabetes. 25-OH Vitamin D2 and 25-OH Vitamin D3 as vitamin D metabolites are measured in human serum to evaluate vitamin D level. Vitamin D supplements are considered as an effective way to treat or to prevent the diseases.
Synonyms THE™ 25-OH Vitamin D3 Antibody (32F9C4), mAb, Mouse
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.