Accelerated apoptosis is associated with many diseases, including AIDS, Alzheimer's and Parkinson's. Decreased apoptosis levels may result in autoimmune disorders and facilitate viral infections. Reliable detection and monitoring of apoptosis is vital in the development of treatments for apoptosis-associated diseases and in the analysis of the effectiveness of compounds that initiate or prevent apoptosis. GenScript's Apoptosis Detection Kits are designed for biomedical research and the efficiency analysis for pro- and anti-apoptotic compounds.
Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
The entire procedure takes about three hours.
Increased Convenience: The results can be observed by fluorescence microscope and light microscope.
1 Kit (100 Assays)
$598.00
1. Detection of apoptotic cells in paraffin-embedded myocardium tissue sections using A. GenScript's TUNEL Apoptosis Detection Kit (L00297) B. Competitor's TUNEL apoptosis detection kit. Arrows indicate apoptotic cells.
Negative control group
Apoptosis-induced group
A. GenScript
B. Competitor
2. Observe and analyze apoptosis by GenScript's Cell Apoptosis PI Detection Kit (L00311): P388 cells are induced apoptosis by 10 μM camptothecin for one hour at 37°C, and procedures are accomplished according with protocol as described as above. The resμlts are as follows:
3. The morphological change of nuclear chromatin in apoptosis observed by Cell Apoptosis DAPI Detection Kit (L00312): P388 cells are induced apoptosis by 10 μM camptothecin for one hour at 37°C, and procedures were accomplished according with protocol as described as above. The result is as follow:
I: Control
II: Stage I
III: Stage IIa
IV: Stage IIb
4. Human P388 cells were stimulated with 4 μM camptothecin for four hours followed by incubation with the reagent in Incubation Buffer for 15 minutes prior to visualization. After washing, cell fluorescence was measured on a flow cytometry and observed with a microscopy (see Figure 1 and 2). Figure 1. Flow cytometer analyzed apoptotic and healthy cells stained with Mitochondrial Apoptosis Detection Kit using FL1 and FL2 channels:
Figure 2. Fluorescence Microscopy analyzed apoptotic and healthy cells stained with Mitochondrial Apoptosis Detection Kit