DTNB

*This product has been discontinued!*

DTNB is known as Ellman reagent. A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.It is used for the colorimetric determination of thiol groups in biological samples. It is fairly soluble in water. Colorless DTNB is converted to yellow 5-Mercapto-2-nitrobenzoic acid in the presence of thiol compounds. As 5-Mercapto-2-nitrobenzoic acid has an abdorption maximum at 412 nm, the absorption spectrum of DTNB does not interfere with thiol detection.

Cat. No.	C01604
Size	Buy in bulk

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Product Introduction

Description

none

Synonyms

5,5-Dithiobis(2-nitrobenzoic acid); 3-Carboxy-4-nitrophenyl disulfide; 6,6'-Dinitro-3,3'-dithiodibenzoic acid; Bis(3-carboxy-4-nitrophenyl) disulfide

Product Introduction

Cas No	69-78-3
Appearance	Pale yellow crystalline powder.
Loss on drying	≤1%
Melting Point	237-247°C
Molar abso	≥1.2x10⁴ (λ_max305 nm)
Molecular Formula	C₁₄H₈O₈N₂S₂
Molecular Weight	396.0
Solubility	10 mg/ml ethanol, 2 mg/ml 50 mM phosphate buffer pH 7.2
UV	A₄₂₀≤0.1%
Storage	Ambient temperature.

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