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PCR Cloning Kit

CloneEZ® PCR Cloning Kit

GenScript'sproprietary CloneEZ® Seamless cloning technology is a cutting-edge, recombination-based cloning technology that can accurately and efficiently assemble genes and gene fragments in any combination of sequences. The CloneEZ® PCR Cloning Kit is designed for the quick cloning of PCR products without any headaches of restriction sites. The universal CloneEZ® cloning method works with any insert and any vector at any restriction site. Using our proprietary CloneEZ® enzyme, this kit rapidly generates precise, directional constructs in 30-min at a room temperature.

PCR cloning kit

CloneEZ® PCR Cloning Kit can be used for all types of cloning that involves the joining of DNA fragments and also has a variety of applications:
  • PCR cloning of up to 12 kb
  • High-throughput (HTP) PCR cloning
  • Gene transfer from one vector to another
  • In vitro joining of DNA fragments

Key Features:

  • Fast and Easy: 30-minute, one-step incubation at room temperature, seamless joints with no extra or unwanted base pairs
  • High Efficiently: Success rate over 95%
  • Universal: Clone any insert into any location, within any vector you choose
  • Compatibility: The kit is highly compatible with high-throughput PCR cloning
  • Products Details
  • FAQs
  • Examples

CloneEZ® PCR Cloning Kit can cut down PCR Cloning procedure to 30 min from traditional 3.5 hrs, with one-step procedure instead of multiple steps.

PCR Cloning Kit

  CloneEZ® Seamless Technology
Classical Cloning
Directional
  • Restriction digestion
  • Gel extraction
  • Ligation
Restriction enzymes not required
X
Ligase not required
X
Reaction Time
0.5 hr
>3.5 hr
Reagents Included
  • CloneEZ® enzyme (50 ul, 5 U/ul)
  • 10X Buffer (100 ul)
  • pUC57 Linear Vector (Kpnl/HindIII, 10 ul, 100 ng/ul)
  • 1 kb contract insert ( 10 ul, 100 ng/ul)
  • Ligase enzyme
  • 10X Buffer
High-efficiency cloning with long fragments (15-20 bp recommend) 4 kb 2 kb 1 kb
NA
93% 95% 100%

Components L00339
CloneEZ® Seamless Enzyme (5 U/μl) 50 μl
10X CloneEZ® Seamless Buffer 100 μl
pUC57 Linearized (EcoRI/HindIII, 30 ng/μl) 10 μl
1-kb Control Insert (100 ng/μl) 10 μl

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00339 CloneEZ® PCR Cloning Kit
24 rxns
$175.00

 
General Questions
  • What are the differences between the CloneEZ® kit and the PCR Cloning Kit?
    A: CloneEZ® Seamless cloning technology offers high efficiency, quick and direct cloning, bypassing all the tedious procedures of traditional cloning that often involve restriction, ligation, and sometimes phosphorylation. This technology works with any DNA sequence and any vector. The only requirement is that your PCR primers have to have a sequence containing 15 or more nucleotides homologous to vector sequence.

  • The technical information in patent document is not available in the manual, why is that?
    A: Currently, the patent on CloneEZ® method is invalid, so we can't provide more details about the patent technical information to the general public, and we will not show more detailed explanation in the manual until the patent is valid.

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A: Shown below is number and percentage of positive clones obtained with the CloneEZ® Kit.

  1 kb 2 kb 4 kb
Number of positive clones ~1200 ~800 221
Recombinant rate (%) 100% 95% 93%

B: Shown below is PCR screening result of positive clones obtained with the CloneEZ® Kit.
PCR cloning kit