Bacterial Expression Systems
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Bacterial protein expression systems are both the most common and the most economical means of protein expression available today. However, two major problems often prevent researchers from making full use of the systems' advantages:
1) The protein of interest cannot always be expressed using the specific vector/strain combination
2) The proteins expressed in bacterial system are sometimes insoluble
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GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins. BacPowerTM technology package includes systematic procedures for expression optimization, such as testing of expression vectors and bacterial strains, induction optimization, and refolding.
- Using BacPowerTM, GenScript can offer customers our Guaranteed Package for protein expression and purification, which guarantees delivery of 3 mg or more of purified soluble protein.
- For proteins that can be easily expressed in bacterial systems, we offer our Standard Package.
- For large-scale protein production, we offer fermentation and large-scale production and purification services.
Guaranteed Package:
| Steps | Deliverables |
| subclone:
Subclone the gene into expression vector(s)
(Customer provides the gene or GenScript synthesizes the gene for an additional fee)
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- Expression construct(s)
- Maps and QC data
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| Expression optimization and verification:
1. Transformation of construct(s) into bacteria
2. Expression optimization: induction time, temperature, and IPTG concentration
3. Coomassie blue stained SDS-PAGE gel
4. Yield estimation and feasibility proposal for large-scale expression (optional)
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- Expression protocols
- Transformed bacterial strain(s)
- Experimental reports and QC data
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| Expression and purification:
1. Expression in 1 L bacterial culture
2. One-step affinity purification
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- 3 mg or more of purified soluble tagged protein
- Experimental reports and QC data
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Standard Package:
If you do not foresee any problems with the expression of your protein, we recommend our Standard Bacterial Expression Package. It includes subcloning the gene into expression construct, transformation of the construct into bacterial strain, protein expression in 1L bacterial culture using our standard induction procedure, and one-step affinity purification. This service can be expanded to include tag removal, endotoxin removal, and additional custom purification for a separate fee. Get a quotation today.
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Large-Scale Production:
Our Large-Scale Expression Package offers protein induction and purification in batches of up to 1,000 L. We can deliver cell pellets or purified recombinant protein. We also offer other services including tag removal, endotoxin removal, and additional custom purification. Contact us for a quotation.
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Quotations and Ordering:
 To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure web server.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.
For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure web server.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: protein@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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