eBlot™ Protein Transfer System

For convenient, fast & cost-effective protein transfer
eblot Protein Transfer· Fast - complete protein transfer in 7 to 10 minutes
· Sensitive - high transfer efficiency as existing methods
· Convenient - no needs for additional power supply and added buffers
· Safe - no methanol required, eliminating special treatment before disposal
· Economical - lower cost per gel than other fast protein blotting systems



  eBlot™ semi-dry blotting system  

   

The eBlot™ Protein Transfer System is based on the proprietary fast semi-dry blotting technique developed by GenScript. To use the eBlot™ Protein Transfer System for rapid protein blotting, assemble the eBlot™ Protein Transfer Pad with your pre-run gel and the membrane on the eBlot™ Protein Transfer Device. A certain definite voltage between electrodes allows for rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane within 7-10 minutes.


protein gel staining

Figure 1. System Mechanism
The eBlot™ Cathode Pad and eBlot™ Anode Pad act as ion reservoirs that contain the appropriate anode and cathode buffers. A certain definite voltage between electrodes drive a rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane.




       protein gel staining
       protein gel staining
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eBlot™ Protein Transfer System
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  • Description
  • Procedures
  • Ordering
  • FAQ
  Shortened workflow increases your productivity 
The eBlot™ Protein Transfer System is faster and provides superior transfer quality compared to the conventional semi-dry western transfer and wet transfer systems (Table 1, Figure 2 and 3).
Table 1. Fast protein transfer with the eBlot™
eblot system
  High and consistent protein transfer efficiency 
In addition to ease and convenience, the eBlot™ Protein Transfer System provides higher detection sensitivity comparable to that of traditional protein transfer methods (Figure 2).
eblot transferred

Figure 2. Higher transfer quality of eBlot™ Protein Transfer System
(A) Comparison of protein transfer between eBlot™ Protein Transfer System and a semi-dry western transfer method. (B) Comparison of protein transfer between eBlot™ Protein Transfer System and a tank wet western transfer method. Proteins were separated with Genscript ExpressPlus™ PAGE Gels 8-16% and transferred to nitrocellulose . (A) Lanes 1-5 and 6-10: 0.313 µl, 0.625 µl, 1.25 µl, 2.5 µl, and 5.0 µl Genscript EasyWestern Protein Standard (22, 40, 60, 85, and 120 kDa); (B) Lanes 1-5 and 6-10: 5.0 µl, 2.5 µl, 1.25 µl, 0.625 µl, and 0.313 µl Genscript High Range EasyWestern Protein Standard (60, 85, 120, 160 and 200 kDa).

  Optimized self-contained system offers easy and convenient procedures 

The eBlot™ Protein Transfer System is created as a self-contained system. All you need to do is to simply assemble the eBlot™ Protein Transfer Pads and membrane together with the pre-run gel, then press the run button (Figure 3).


eblot Protein transfer System
Figure3. eBlot™ Protein Transfer System
  Suitable for various types of mini gels 

The eBlot™ Protein Transfer System is designed to work with multiple types of mini polyacrylamide gels, including Tris-Glycine, Bis-Tris, Tris-Tricine, and Tris-Acetate gels, etc.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L03010 eBlot Protein Transfer Device
1 unit
$899.00
L03011 eBlot Protein Transfer Pads (Basic, 20-pak)
1 box
$79.00
L03013 eBlot Protein Transfer Pads (Nitrocellulose, 20-pak)
1 box
$159.00
L03014 eBlot Protein Transfer Pads (PVDF, 20-pak)
1 box
$169.00
L03012 eBlot Graphite Electrode
1 unit
$59.00
M01078 eBlot Equilibration Buffer
125 ml
$59.00

*Customers buy one unit eBlot™ Protein Transfer Device (L03010) and get one box of eBlot™ Protein Transfer Pads (Nitrocellulose, 20-pak) (L03013) at $79(over 50% off) or eBlot™ Protein Transfer Pads (Basic, 20-pak) (L03011) for free.

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  1. What's the difference between eBlot™ Protein Transfer System and conventional blotting methods?
  2. Which types of protein gels are eBlot™ Protein Transfer System compatible with?
  3. How many gels can be blotted at the same time?
  4. What are the functions of eBlot™ Protein Transfer Pads?
  5. Which eBlot™ Protein Transfer Pads should I choose?
  6. Can the eBlot™ Protein Transfer Pads be re-used for blotting multiple gels?
  7. Why should I replace the eBlot™ Graphite Electrode and how often should I do it?
  8. Can I blot very thin (less than 1 mm) or very thick (more than 1 mm) gels using eBlot™ Protein Transfer System?
  9. What is the recommended temperature for using eBlot™ Protein Transfer System?
  10. Why is there a small crack at the titanium cathode side of the lid of the eBlot™ Protein Transfer device after a period of use?
  11. Can I start a run without the assembled transfer stack (eBlot™ Anode Pad-Membrane-Gel-Gel Window-eBlot™ Protein Transfer Cathode Pad) in place?

Answers:

  1. What's the difference between eBlot™ Protein Transfer System and conventional blotting methods?
  2. A: Conventional wet and semi dry blotting processes take hours to complete, results can be unpredictable. The eBlot™ Protein Transfer System applies GenScript's proprietary electric transfer technology, where a certain definite voltage is applied to the graphite anode and titanium cathode to drive the negatively charged protein from mini polyacrylamide gels onto membrane, therefore, significantly reduces the time required for protein transfer, from hours down to 7 to 10 minutes. .

  3. Which types of protein gels are eBlot™ Protein Transfer System compatible with?
  4. A: The eBlot™ Protein Transfer System is designed to work with multiple homemade or precast mini polycrylamide gels, including Tris-Glycine, Bis-Tris, Tris-Acetate and Tris-Tricine gels, etc. For specially formulated gels, optimization of the transfer time may be needed for best results.

  5. How many gels can be blotted at the same time?
  6. A: One mini gel can be blotted per run.

  7. What are the functions of eBlot™ Protein Transfer Pads?
  8. A: The eBlot™ Protein Transfer Pads are the consumable part of eBlot™ Protein Transfer System. Each pack of eBlot™ Protein Transfer Pad contains an eBlot™ Cathode Pad (white) and Anode Pad (yellow) presoaked with proprietary buffers, acting as ion reservoirs.

  9. Which eBlot™ Protein Transfer Pads should I choose?
  10. A: We offer transfer pads coming with membrane: Nitrocellulose (cat. no. L03013) or PVDF (cat. no.L03014). If you already have your own membrane, you can choose our basic package without membrane (cat. no. L03011).

  11. Can the eBlot™ Protein Transfer Pads be re-used for blotting multiple gels?
  12. A: No. For best results, one pack of eBlot™ Protein Transfer Pad is used for one gel. Discard used eBlot™ Protein Transfer Pads after each blotting.

  13. Why should I replace the eBlot™ Graphite Electrode and how often should I do it?
  14. A: During electric transferring process, the eBlot™ Graphite Electrode will absorb ions from anode pad while lose carbon composition, which will change the conductivity of the graphite electrode, thereby affecting the blotting results. For best results, after 100 uses, the worn eBlot™ Graphite Electrode should be replaced by a new one; the graphite electrode should also be replaced when blotting performance is significantly reduced.

  15. Can I blot very thin (less than 1 mm) or very thick (more than 1 mm) gels using eBlot™ Protein Transfer System?
  16. A: Yes. However, the transfer time needs to be optimized. Also, for 0.75-1mm thickness gels, use 2 pieces of sponge cushion underneath the electrode; for 1.5 mm gels, use only 1 piece of sponge cushion instead.

  17. What is the recommended temperature for using eBlot™ Protein Transfer System?
  18. A: For best staining results, it is recommended to use the system at room temperature (22-28 ℃). If temperature is below 22 ℃, users may need to extend the running time to obtain satisfied results based on gel thickness.

  19. Why is there a small crack at the titanium cathode side of the lid of the eBlot™ Protein Transfer device after a period of use?
  20. A: A tiny crack may develop at one of the two lower corners next to the titanium cathode due to thermal expansion difference between the lid material and the titanium cathode. However, it does not affect the transfer performance of the device.

  21. Can I start a run without the assembled transfer stack (eBlot™ Anode Pad-Membrane-Gel-Gel Window-eBlot™ Protein Transfer Cathode Pad) in place?
  22. A: No. It will DAMAGE the device due to short circuit.

Troubleshooting

Problem
Cause
Solution
The right Status Light doesn't flash during blotting process.
Incomplete electric circuit due to improper assembly of the transfer pads.
Ensure the transfer stack is assembled correctly: use the eBlot™ Anode Pad first followed by membrane, the pre‐run gel, Gel Window and eBlot™ Cathode Pad.
The left and right Status Lights flash simultaneously.
Excessive current is flowing through the Device.
Check the transfer stack and ensure Gel Window covered correctly on the gel.
Inefficient transfer Empty spots on the membrane
1. Salt built‐up on plate electrodes
1. Clean the titanium cathode plate, graphite anode plate with a wet cloth or paper tissue followed by a dry one to remove any insoluble salts.
2. Membrane insufficiently equilibrated in eBlot™ Equilibration buffer
2. Equilibrate membrane in eBlot™ Equilibration buffer before transfer.
3. Incorrect transfer conditions or insufficient transfer time
3. Use a gel of lower concentration to separate high molecular weight proteins. Increase the transfer time in 5‐second increments.
4. PVDF membrane was not prewet with methanol
4. Pre‐wet PVDF membrane with methanol before transfer.
5. Confusion of the eBlot™ Anode Pad and Cathode Pad Air bubbles trapped between gel and membrane prevent the transfer of proteins
5. Ensure the transfer stack is assembled correctly: Bottom‐eBlot™ Anode Pad (yellow), Top‐eBlot™ Cathode Pad (white). When assembling transfer stack, use the small shovel supplied with the device to remove any air bubbles between the gel and the membrane