FAQ for Peptide - GenScript

Email: info@genscript.com Tel: 1-732-885-9188 Secure Online Message

Frequently Asked Questions: custom peptide synthesis

1. What is the typical turnaround time for peptide synthesis?
2. How may I place my order?
3. How much does it cost to ship a peptide outside the US?
4. What kinds of payment do you accept?
5. How do you ship peptides? What data will be provided?
6. How pure does my peptide need to be?
7. What methods do you use to purify the peptide?
8. What is the purity level of the crude peptide?
9. What is the purity level of the desalted peptide?
10. What methods do you use?
11. How should I store the peptides?
12. What is the best way to dissolve the peptide?
13. Is there any limitation on the length of peptide that GenScript can synthesize?
14. What if there is a problem with the synthesis of my peptide?

1. What is the typical turnaround time for peptide synthesis at GenScript?
Our typical turnaround time is about three weeks. The turnaround time may vary depending on the peptide length and complexity of synthesis.

2. How may I place my order?
GenScript needs the sequence, desired purity, desired quantity, and preferred payment information. If you have a PO (Purchase Order) number or credit card, you can place the order directly online using our secure server. Alternatively, you can send us the sequence by email and fax us the PO. If you like, you may view Instant Online Quotations for all peptide orders up to 1000 mg.

3. How much does it cost to ship a peptide outside the US?
We ship our products to many countries. The overseas shipping charge is $25 per shipment for most countries.

4. What kinds of payment do you accept?
GenScript accepts credit cards (Visa, MasterCard, American Express), checks, and wire transfers. For orders, you may send us a PO (Purchase Order). For certain orders, GenScript may require a deposit.

5. How do you ship peptides? What data will be provided?
All peptides will be lyophilized and shipped in small microcentrifuge tubes (2 ml). Large amounts of peptide may be aliquoted into several tubes. For every single peptide, data sheets containing information such as amino acid sequence, modifications, purity, mass spectrum data, and HPLC data will be provided.

6. How pure does my peptide need to be?
That depends on your specific application. GenScript can synthesize peptides to 98% purity. Here is a general guideline for peptide purity requirements:

Purity	Application
>85%	Immunological applications and polyclonal antibody production
>90%	SAR studies, bioassays
>95%	In vitro bioassays such as ELISA, enzymology, biological activity
>98%	NMR, crystallography, sensitive bioassays

7. What methods do you use to purify the peptide?
HPLC is used for purification.

8. What is the purity level of the crude peptide?
Generally 50-60%.

9. What is the purity level of the desalted peptide?
Generally 60-70%.

10. What methods do you use to synthesize peptides?
For peptides with fewer than 50 amino acids, we use stepwise SPPS chemical methods. For peptides with more than 50 amino acids, we use fragment condensation, native ligation or our proprietary recombinant methods.

11. How should I store the peptides?
Lyophilized peptides can be stored long-term at -20℃.

12. What is the best way to dissolve the peptide?
The solubility of given peptides varies depending on their amino acid sequences and modifications. GenScript purifies peptides by HPLC using a water and acetonitrile gradient. Here are some general tips for dissolving peptides:

o Sonication will increase solubility.
o 10% acetic acid in the solvent will help dissolve basic peptides.
o 10% ammonium bicarbonate will help dissolve acidic peptides.
o For peptides with extremely low solubility in aqueous solutions, organic solvents (such as DMSO, isopropanol, methanol, and acetonitrile) should be used first. Once the peptides are completely dissolved, water may be gradually added until the desired concentration is obtained.

13. Is there any limitation on the length of peptide that GenScript can synthesize?
None whatsoever. GenScript can synthesize peptides of any length by using chemical or recombinant methods.

14. What if there is a problem with the synthesis of my peptide?
Each peptide has its specific characteristics and not all outcomes can be anticipated. If something goes wrong during the synthesis and we cannot deliver your peptide on time, we will inform you as soon as possible.