PCR Cloning? Gene Synthesis!
| PCR cloning
is the most powerful genetic research and biotechnology technique
developed in recent years. Its impact on the many areas of molecular
biology, cloning, and genetics cannot be underestimated. With this
technique, a target DNA sequence can be amplified a billionfold in a
matter of hours. Within the past few years, scientists have extended
the benefits of PCR cloning from the basic research laboratory into drug development, forensics, agricultural management, and far beyond.
One of the biggest influences on biological and genetic research today is the flood of genomic-sequence data pouring into databanks public and private. As the amount of genetic information increases, demands to process it into useful applications become at once more pressing and more difficult to fulfill. Although the basics of PCR are relatively easy to master, even for undergraduate students, some of the newer PCR-based applications are quite tricky and laborious, and many people have difficulty obtaining reproducible results. PCR cloning has its faults, such as contamination, non-specific products, and primer dimers, which all make PCR cloning time- and energy-consuming. GenScript gene synthesis technology is currently the fastest and most efficient means available for the creation and optimization of DNA sequences, and it sidesteps most of the problems inherent in PCR cloning. Our codon optimization process gives us an undeniable advantage over our more expensive competitors. Because gene synthesis is so essential to gene expression, recombinant protein expression projects, and template amplification (especially high-GC template and long PCR amplification), a system that can provide the lowest mutation rate possible is similarly vital. GenScript's mutation rate is near zero. In addition, gene synthesis is often far more affordable than traditional PCR cloning. Please view the cost estimates below. |
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Typical PCR Cloning Project: |
| 1. One marathon-ready cDNA library: 1 x $510 = $510
2. Correction of three SNP mutations: 3 x $400 = $1200 3. PCR cloning kit, primers, sequencing, enzymes, and other reagentst: $700 Total cost: $2410 |
| Even at a cost of $2410, PCR cloning cannot guarantee the delivery optimize codon usage the same way that GenScript gene synthesis can. GenScript's high-quality, competitive gene synthesis services start as low as $0.66 per base pair. GenScript's custom gene synthesis offers the quickest and most cost-effective means of obtaining any gene you may require. |
Quotations and Ordering: |
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| Orders can be placed by phone, , fax or online with a formal PO (Purchase Order) or credit card. Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. For privacy protection, please submit your gene sequences via our secure online ordering system. | ||||||||
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