dna polymerase

Green PCR Reagents

GenScript's advanced high-stability technology has led to the invention of the new generation of PCR reagents—Green PCR Reagents. The Green series of reagents, from the proprietary Taq, dNTP, to PCR mixtures, are stable for one month if stored at room temperature, or for over six months if stored at 4°C. The high-stability feature comes with increased half-life of active enzymes at high temperatures, which results in more amplification cycles and higher yield. With no freeze-thaw cycles, green PCR reagents provide you an instant access to high-throughput PCR automation. In addition, GenScript's Green PCR Reagents can help preserve energy associated with storage and shipping.

Key Features

  • Stable at room temperature
  • Enhanced yields with longer enzyme half-life
  • Stop refrigerating and save energy
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  • Examples
  • Product Resources
  • Related Products

Green Taq DNA Polymerase

Green Taq DNA Polymerase has terminal transferase activity that results in the addition of single nucleotide (adenosine) at 3'-end of the extension product. This enzyme also has a longer enzyme half-life and therefore increases the PCR yields when amplifying long DNA fragments.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
E00043 Green Taq DNA Polymerase
1000 U
$60.00

High-Stability PCR Kit

GenScript's High-Stability PCR Kit contains Green Taq DNA Polymerase, 10X PCR buffer, and 10 mM dNTP, all specially formulated for higher stability using our proprietary technology. The stabilizers in the storage buffer also increase the stability of Taq DNA Polymerase at higher temperatures (72°C), increasing the PCR yield of long DNA amplification. This Kit makes the PCR step quick and easy by eliminating any need for thawing. This kit can be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR at any time.

Ordering Information

Cat. No.
Product Name
Size
Price
Quantity
L00342 High-Stability PCR Kit
1 kit
$64.00

Specification of High-Stability PCR Kit

Components Volume Documents
Green Taq DNA polymerase 100 μl dna polymerase
10X reaction buffer 1.5 ml
Stabilized dNTP 300 μl
Protocol 1
 

  • The Green Taq DNA polymerase provides high PCR yield and specificity when amplifying long DNA fragments.
  • dna polymerase

    Figure 1. An 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and Green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (In this case, the elongation time was eight minutes.), the Green Taq DNA polymerase has a longer half-life and therefore increases the PCR DNA yields significantly.

  • The Green Taq DNA polymerase can be stored at 37°C for 14 days without activity loss.

    dna polymerase

  • Figure 2. A 2-kb 23S rRNA fragment from from E. coli genomic DNA was amplified using Green Taq DNA polymerase stored at -20°C and 37°C, respectively.


    Citations  

    Ordering Information

    Cat. No.
    Product Name
    Size
    Price
    Quantity
    E00007-50000 Taq DNA Polymerase
    50000 U (50 x 1000 U)
    $2700.00
    E00007-1000 Taq DNA Polymerase
    1000 U
    $60.00
    E00008 Taq DNA Polymerase without Mg2+
    1000 U
    $60.00
    C01689 Stabilized dNTP Mix, 10 mM each
    0.500 ml
    $29.00
    E00019 2X Taq Master Mix
    100 Reactions
    $43.00
    D0056 dNTP mixture, 10 mM each
    0.500 ml
    $25.00
    E00101 Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml)
    1 PK
    $78.00
    C01577-50 dATP (100 mM) ≥99% HPLC
    50 ml(2x25 ml)
    $1600.00
    C01577-250 dATP (100 mM) ≥99% HPLC
    250 ml
    $7500.00
    C01577-10 dATP (100 mM) ≥99% HPLC
    10 ml
    $360.00
    C01577-1 dATP (100 mM) ≥99% HPLC
    1 ml
    $45.00
    C01578-50 dGTP (100 mM) ≥99% HPLC
    50 ml(2x25 ml)
    $1600.00
    C01578-250 dGTP (100 mM) ≥99% HPLC
    250 ml
    $7500.00
    C01578-10 dGTP (100 mM) ≥99% HPLC
    10 ml
    $360.00
    C01578-1 dGTP (100 mM) ≥99% HPLC
    1 ml
    $45.00
    C01579-50 dCTP (100 mM) ≥99% HPLC
    50 ml(2x25 ml)
    $1600.00
    C01579-250 dCTP (100 mM) ≥99% HPLC
    250 ml
    $7500.00
    C01579-10 dCTP (100 mM) ≥99% HPLC
    10 ml
    $360.00
    C01579-1 dCTP (100 mM) ≥99% HPLC
    1 ml
    $45.00
    C01580-50 dTTP (100 mM) ≥99% HPLC
    50 ml(2x25 ml)
    $1600.00
    C01580-250 dTTP (100 mM) ≥99% HPLC
    250 ml
    $7500.00
    C01580-10 dTTP (100 mM) ≥99% HPLC
    10 ml
    $360.00
    C01580-1 dTTP (100 mM) ≥99% HPLC
    1 ml
    $45.00
    C01582-50 dNTP (10 mM each) ≥99% HPLC
    50 ml(2x25 ml)
    $720.00
    C01582-250 dNTP (10 mM each) ≥99% HPLC
    250 ml
    $2500.00
    C01582-10 dNTP (10 mM each) ≥99% HPLC
    10 ml
    $154.00
    C01582-1 dNTP (10 mM each) ≥99% HPLC
    1 ml
    $45.00