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Green PCR Reagents
GenScript's advanced high-stability technology has led to the invention of the new generation of PCR reagents---Green PCR Reagents. The Green series of reagents, from the proprietary Taq, dNTP, to PCR mixtures, are stable for one month when stored at room temperature, and for over six months when stored at 4°C. The high-stability feature allows the increase in the half-life of active enzymes at high temperatures, therefore more amplification cycles can be carried out and higher yield generated. With no freeze-thaw cycles, green PCR reagents provide you an instant access to high-throughput PCR automation. In addition, GenScript's Green PCR Reagents can help preserve energy in storage and shipping. |
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Components |
High-Stability PCR Kit
L00342 |
Documents |
| Green Taq DNA polymerase |
100 μl |
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| 10X reaction buffer |
1.5 ml |
| Stabilized dNTP * |
300 μl |
| Protocol |
1 |
| * Free samples available |
Green Taq DNA polymerase increases PCR yield when amplifying long DNA.
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| Figure 1. A 8-kb fragment from λDNA was amplified by using both Taq DNA polymerase and Green Taq DNA polymerase. For long DNA fragment amplification, where greater elongation time is needed, the Green Taq DNA polymerase has a longer half-life and increases the PCR DNA yield significantly. In this case, the elongation time was eight minutes. |
Green Taq DNA polymerase can be stored at 37°C for 14 days without any significant loss of activity.
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Figure 2. A 2-kb 23S rRNA fragment from from E. coli genomic DNA was amplified using Green Taq DNA polymerase stored at -20°C and 37°C, respectively. The Green Taq DNA polymerase can be stored at 37°C for 14 days without any significant loss of activity. |
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| For optimal amplification and detection results, you need products guideline you can rely on. GenScript provides the best range of products, from PCR enzymes to service systems and other reagents.
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