BacPower™ Guaranteed Package Promotion
Our popular
BacPower™ Bacteria Expression Platform is getting more sophisticated! We now fully guarantee both expression and purity of your protein with our 4 free assurance: FREE Gene synthesis , FREE codon optimization, FREE subcloning, FREE to you if the project fails. Simply send us your protein sequence and let us do the rest. We offer guaranteed protein customization options letting you choose your desired purity, either 75% or 85%, as well as the option of tag-free proteins. We guarantee delivery of 3mg of your custom protein and offer easy and inexpensive options to add more to your order. Place your order today to experience unprecedented low pricing exclusively offered with BacPower™ Guaranteed Package.
BacPower™ Guaranteed Package with unprecedented assurance
- FREE Gene synthesis
- Guarantees the amount of custom protein - or it's FREE*
- Guarantees the purity of custom protein - or it's FREE*
- Guarantees custom protein is tag free - or it's FREE*
- Competitive price: starting from $2,200
- Fast turnaround in as little as 6 weeks
* Customized protein services apply if the target protein is greater than 100KDa
- Service specifications
- Case Studies
- Customer Testimonial
- Terms and Conditions
| Customers provide: |
Deliverables |
Timeline |
Promotional price |
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Target protein sequence |
- Optimized gene sequence report
- Guaranteed 3mg protein with 75% or 85% purity
- QC data (SDS-PAGE & Western Blot)
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- 6-10 weeks for
tagged protein
- 8-12 weeks for
tag-free protein
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Starting from $2,200 |
1. Protein refolding
Overproduction of proteins in E.coli can cause the formation of inactive, misfolded and insoluble protein aggregates. GenScript provides proprietary refolding technology to solve this problem. A case study shows that over 95% of inclusion bodies are solublized and refolded. The purity of the refolded protein is more than 85% and is delivered in customized buffer.
Fig. A: Refolded protein of sample was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining
Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat. No. MM0900)
Lane 1: Refolded protein of sample
2. Tag removal
Presence of epitope tag in recombinant proteins may result in changes in protein structure, toxicity, and loss of bioactivity. To avoid these problems, tag removal after protein expression is requested by some customers as an additional service. Shown below is protein that is cleaved by SUMO protease and successfully isolated. Recovered target protein is more than 90% pure and 80% tag free.
Fig. B:
Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat.No. MM0900)
Lane 1: Refolded fusion protein with SUMO-tag
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Fig. C:
Lane M: Smart Protein Standard (Middle-Range) (GenScript, Cat.No. MM0900)
Lane 1: Recovered protein after 1st incubation with Ni-resin
Lane 2: Recovered protein after 2nd incubation with Ni-resin
Lane 3: Elute with 300 mM imidazole from Ni-resin of 2nd incubation |
Customer Testimonial
"GenScript provides fast, professional protein synthesis services at very reasonable prices. By making it cost-effective to outsource protein production, GenScript has made it possible for my lab to focus on our own area of expertise and get more research done. The detailed planning, updates, and reports that GenScript provides all of the quality control that one could ask for. I strongly recommend GenScript's protein production service."
— Dr. Barry Bradford, Kansas State University Department of Animal Sciences & Industry
Partial publications cited our protein services
More publications
Protein sequences are subject to pre-project analysis. Exclusions are applicable for membrane proteins and toxic proteins.
For eligible proteins, a minimum of 3mg soluble protein with 75% or 85% purity will be delivered to customers. The protein services will be free of charge if GenScript's evaluation reveals that the expression level is insufficient to eventually obtain 3mg of purified protein. To increase the expression level of your protein, gene optimization using our OptimumGene™ technology is recommended. Up to 3 fusion tag(s) may be tested to increase the expression level and solubility of the protein and are applied for protein production and purification. Fusion tags may be removed by introducing an optional protease cleavage site between the tag and the target protein (note: tag removal service is offered at extra cost) . Insoluble proteins will be subject to refolding using our FoldArt™ technology at no additional fee.
* Customized protein services apply if the target protein is greater than 100KDa
Quotations and Ordering:
To request a quotation, please fill in the Quotation Form and send it to us by email or fax. Download the Quotation Form in following list:
- For BacPower™ Customized Protein Service (SC1318), please download the
;
- For BacPower™ Guaranteed Package, please download the
.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.
For questions or to inquire about the status of your order, contact us by email, phone, fax or via our secure online messaging system.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: protein@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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